mitochondrial protein synthesis assay

(C) Mitochondrial protein synthesis in COX6C- and SCO2-depleted cells, and in Surf1 −/− HEK293T cells. The Mitochondrial Protein IP kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Mitochondrial protein synthesis can be analyzed in cultured cells by metabolic labeling with ... MEFs were metabolically labeled as described for the mitochondrial translation assays using the second approach. In brief, 400 µg of protein was resuspended in 10-mM Hepes, pH 7.4, to which an equal volume of 200-mM Na 2 CO 3 was added, and then mixed and incubated on ice for 30 min.

Benador et al. A mitochondrial protein-synthesis assay showed reduced levels of respiratory chain subunits in myotubes generated from patient cell lines.

With the assay conditions established as in Fig. Mitochondrial protein synthesis is membrane bound 1218 Channeling of mRNAs to the inner membrane 1219 Localization of protein synthesis by mRNA-specific translational activators 1219 Assembly of cytochrome c oxidase 1220 Assembly of the bc 1 complex 1221 Assembly of the ATP synthase 1222 Perspective 1223 T O think about how mitochondrial proteins are synthe … Our MitoBiogenesis assay measures the levels of two mitochondrial proteins COX-1 and SDH-A simultaneously, making it an ideal tool for uncovering chronic effects of the test compound on mitochondrial DNA replication and protein synthesis. Measurement of mitochondrial biogenesis is becoming a standard part of drug safety characterization.

Picchioni et al.

Others have proposed that the prodrug moiety released from the ribonucleoside analogs might instead cause toxicity. 1 Certain drugs are able to affect mitochondrial biogenesis through inhibition of mtDNA replication or mitochondrial encoded protein synthesis. Mitochondrial health assay images HepG2 cells stained with Invitrogen Hoechst 33342 stain to provide nuclear segmentation for automated cell counting and measures of nuclear morphology.

Click here to … Mitochondrial Protein Synthesis and mtDNA Levels Coordinated through an Aminoacyl-tRNA Synthetase Subunit Daria Picchioni,1,9 Albert Antolin-Fontes,1,9 Noelia Camacho,1 Claus Schmitz,2 Alba Pons-Pons,1 Marta Rodrı´guez-Escriba`,1 Antigoni Machallekidou,1 Merve Nur Guler,€ 1 Panagiota Siatra,1 Maria Carretero-Junquera,1 Alba Serrano,1 Stacy L. Hovde,3 Philip A. Knobel,1,4 Eva M. … Some have hypothesized that the active metabolites of toxic ribonucleoside analogs, the triphosphate forms, inadvertently target human mitochondrial RNA polymerase (POLRMT), thus inhibiting mitochondrial RNA transcription and protein synthesis. Mitochondrial biogenesis. Figure 2 shows the effect of T-2 mycotoxin on mitochondrial protein synthesis.

Samples … Peridroplet mitochondria have enhanced bioenergetic capacity, reduced β-oxidation capacity, support lipid droplet expansion by providing ATP for triacylglyceride synthesis, and maintain a distinct protein composition due to low fusion-fission dynamics. Ageing is associated with mitochondrial degeneration. report on a distinct mitochondrial population associated with lipid droplets in the interscapular brown adipose tissue. In yeast, reducing cytosolic protein synthesis suppresses age-dependent mitochondrial dysfunction and extends lifespan. SLIMP also interacts with a major mitochondrial protease to control mitochondrial DNA levels. Invitrogen Image-iT DEAD Green stain used to label dead cells and provide a viability determination based on cell membrane permeability. report the architecture of a housekeeping enzyme that is essential for mitochondrial protein synthesis.