Protein Source: 30-50g of any medium to fast-acting protein source.
Furthermore, as the proteomics experiment does not capture all proteins, the uncaptured fraction had to be taken into account. Whole-food is okay, but you may want to restrict whole-food protein closer to 60 minutes out than 30 minutes out.
Dose-response curves performed in duplicate. All components of protein function are defined by the exact composition, structure and conformation of the proteins, which is encrypted within the DNA region (called locus) encoding that protein. Cells were washed, fixed, permeabilized, and nascent protein synthesis was detected following a click reaction with Alexa Fluor® 488 alkyne. To obtain absolute protein synthesis rates, the relative rates obtained from pSILAC were scaled, using the known values of the number of protein molecules per yeast cell , the doubling time of yeast, and the average half-life of yeast proteins . Check out the worksheet that goes along with the game, courtesy of Ms. Julie Olson! Examples of fast-acting protein sources include mixtures of whey and casein isolates/hydrolysates and concentrates like Metabolic Drive® Low Carb. It was found that the addition of protein to the carbohydrate supplement increased the rate of glycogen storage by approximately 38% over the first 4-hours of recovery. The greater rate of synthesis was believe due to a greater insulin response as a result of the addition of protein to the carbohydrate supplement (Pallotta and Kennedy, 1968 ; Spiller et al., 1987 ).
With the process of protein synthesis biological cells generate new proteins, which on the other hand is balanced by the loss of cellular proteins via degradation or export. Because each ribosome is producing one protein molecule, the rate of protein synthesis is proportional to the ribosome density of a given gene as measured by the footprint density (number of footprint per unit length of the gene), provided that all ribosomes complete a full length protein and have similar average rates of elongation across genes. U2OS cells were treated in L-methionine-free media and 50 μM Click-iT AHA for 30 minutes with anisomycin (17 pM–1 μM), cycloheximide (85 pM–5 μM), or puromycin (1.7 nM–100 μM). Topics Covered: Protein synthesis, transcription, translation, amino acids, ribosomes, tRNA, mRNA, nucleotides etc.